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nested polymerase chain reaction (pcr)  (Illumina Inc)


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    Structured Review

    Illumina Inc nested polymerase chain reaction (pcr)
    Nested Polymerase Chain Reaction (Pcr), supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nested+polymerase+chain+reaction+(pcr)/nested+polymerase+chain+reaction++pcr+/10__1002_slash_lno__11727-113-17-32
    Average 90 stars, based on 1 article reviews
    nested polymerase chain reaction (pcr) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Whole Complete genome sequencing:

    Article Title: Monitoring antimicrobial resistance trends and emerging carbapenemases in Enterobacterales causing companion animal infections: a four-year study.
    Article Snippet: DNA for genomic applications was extracted using the NZY Tissue gDNA Isolation kit (NZYTech, Lisbon, Portugal) following the standard protocol for bacteria, aside from an extension of the proteinase K lysis time to be performed overnight and applying the optional RNAse treatment. .. Library preparation and WGS were performed by Macrogen (Seoul, Republic of Korea), using the Illumina TruSeq DNA PCR-free preparation kit and the Novaseq platform with 2 × 150 bp paired-end reads. ..

    Polymerase Chain Reaction:

    Article Title: Monitoring antimicrobial resistance trends and emerging carbapenemases in Enterobacterales causing companion animal infections: a four-year study.
    Article Snippet: DNA for genomic applications was extracted using the NZY Tissue gDNA Isolation kit (NZYTech, Lisbon, Portugal) following the standard protocol for bacteria, aside from an extension of the proteinase K lysis time to be performed overnight and applying the optional RNAse treatment. .. Library preparation and WGS were performed by Macrogen (Seoul, Republic of Korea), using the Illumina TruSeq DNA PCR-free preparation kit and the Novaseq platform with 2 × 150 bp paired-end reads. ..

    Article Title: Donor-derived CD8 + CD122 + Tregs generated in mixed donor chimeric NOD mice delete autoreactive T cells
    Article Snippet: RNA libraries were prepared using the Takara SMART-Seq mRNA Kit (Cat. #634773) following the manufacturer’s instructions. .. Libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, Cat. #FC-131-1096) and indexed via tagmentation and PCR amplification to generate Illumina-compatible libraries. .. Indexed libraries were pooled, normalized to 4 nM, and sequenced on an Illumina NovaSeq X Plus platform using paired-end (2×100 bp) reads.

    Article Title: Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.
    Article Snippet: .. Second-strand synthesis incorporateddUTP to preserve strandedness, followed by A-tailing, ligation of TruSeq adapters with unique dual indexes, and limited-cycle PCR (TruSeq Stranded mRNA Library Prep, Illumina #20020595). .. Libraries were purified with AMPure XP beads, QC’d by Bioanalyzer DNA1000 or FragmentAnalyzer, quantifiedbyqPCR (KAPAKK4835) and sequenced 1 * 51 + 10 + 10 bp on Illumina’s NextSeq2000.

    Article Title: Exploring contributions and responses of microbial quantity and diversity to coastal seasonal hypoxia.
    Article Snippet: Dissolved oxygen (DO) concentrations in euphotic waters are governed by combined effects from air-sea exchange, photosynthesis, and aerobic respiration.. Bacteria and microeukaryotes both respond to and shape these oxygen dynamics, yet the quantitative contributions of microbial traits versus physicochemical factors to DO variability remain unclear.. Moreover, the extent to which DO, and other environmental parameters or interdomain biotic interactions control microbial diversity and abundance is still unresolved.

    Article Title: Genome Sequencing and Genome-Wide Analysis Uncover Candidate Genes and Loci Linked to Key Production Traits in the Philippine Black Tiger Shrimp (Penaeus monodon).
    Article Snippet: Penaeus monodon (black tiger shrimp) is a key species in aquaculture globally and in the Philippines.. The selection and development of stocks with desirable production traits, such as enhanced growth and disease resistance, are critical for improving production.. In this study, we leveraged a newly assembled, highquality genome sequence from a Philippine population to perform genomewide scans for SNPs associated with key production traits.

    Article Title: The pan-genome provides insights into evolutionary dynamics and fatty acid metabolism in Aceraceae family.
    Article Snippet: .. Library preparation was performed using the Illumina TruSeq DNA PCR-Free library kit to ensure high-quality, adapter-ligated fragments suitable for sequencing. .. The prepared libraries were then subjected to paired-end sequencing using the Illumina HiSeq X Ten platform, producing high-coverage (average 30x) reads.

    Article Title: Centromeric retrotransposons shape chromosomal evolution beyond pericentromeric regions.
    Article Snippet: .. Short-read whole-genome sequencing (used for polishing ONT contigs) was performed at the Next Generation Sequencing (NGS) facility of Bern, Switzerland (https://www.ngs.unibe.ch/), using the Illumina TruSeq DNA PCR-free kit for library preparation and 150bp paired-end reads (insert size of 550 bp) for sequencing on Illumina NovaSeq 6000 (estimated sequencing depth of 40X). .. Hi-C sequencing of three species (B. varia, B. prealpina and B. frutescens) was carried out by Phase Genomics company (Seattle, USA).

    Article Title: A grafting-rescued albino mutant reveals the essential function of CspTAC12 in cucumber chloroplast biogenesis.
    Article Snippet: Chloroplast biogenesis requires precise coordination between the nuclear and plastid genomes, orchestrated by the plastid-encoded RNA polymerase (PEP) complex.. In this study, we characterized a spontaneous albino lethal mutant, XNS106_1, in cucumber.. The mutant exhibits complete chlorophyll deficiency and photosynthetic failure, leading to post-germinative lethality.

    Construct:

    Article Title: Donor-derived CD8 + CD122 + Tregs generated in mixed donor chimeric NOD mice delete autoreactive T cells
    Article Snippet: RNA libraries were prepared using the Takara SMART-Seq mRNA Kit (Cat. #634773) following the manufacturer’s instructions. .. Libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, Cat. #FC-131-1096) and indexed via tagmentation and PCR amplification to generate Illumina-compatible libraries. .. Indexed libraries were pooled, normalized to 4 nM, and sequenced on an Illumina NovaSeq X Plus platform using paired-end (2×100 bp) reads.

    Article Title: A grafting-rescued albino mutant reveals the essential function of CspTAC12 in cucumber chloroplast biogenesis.
    Article Snippet: Chloroplast biogenesis requires precise coordination between the nuclear and plastid genomes, orchestrated by the plastid-encoded RNA polymerase (PEP) complex.. In this study, we characterized a spontaneous albino lethal mutant, XNS106_1, in cucumber.. The mutant exhibits complete chlorophyll deficiency and photosynthetic failure, leading to post-germinative lethality.

    DNA Library Preparation:

    Article Title: Donor-derived CD8 + CD122 + Tregs generated in mixed donor chimeric NOD mice delete autoreactive T cells
    Article Snippet: RNA libraries were prepared using the Takara SMART-Seq mRNA Kit (Cat. #634773) following the manufacturer’s instructions. .. Libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, Cat. #FC-131-1096) and indexed via tagmentation and PCR amplification to generate Illumina-compatible libraries. .. Indexed libraries were pooled, normalized to 4 nM, and sequenced on an Illumina NovaSeq X Plus platform using paired-end (2×100 bp) reads.

    Amplification:

    Article Title: Donor-derived CD8 + CD122 + Tregs generated in mixed donor chimeric NOD mice delete autoreactive T cells
    Article Snippet: RNA libraries were prepared using the Takara SMART-Seq mRNA Kit (Cat. #634773) following the manufacturer’s instructions. .. Libraries were constructed using the Nextera XT DNA Library Preparation Kit (Illumina, Cat. #FC-131-1096) and indexed via tagmentation and PCR amplification to generate Illumina-compatible libraries. .. Indexed libraries were pooled, normalized to 4 nM, and sequenced on an Illumina NovaSeq X Plus platform using paired-end (2×100 bp) reads.

    Ligation:

    Article Title: Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.
    Article Snippet: .. Second-strand synthesis incorporateddUTP to preserve strandedness, followed by A-tailing, ligation of TruSeq adapters with unique dual indexes, and limited-cycle PCR (TruSeq Stranded mRNA Library Prep, Illumina #20020595). .. Libraries were purified with AMPure XP beads, QC’d by Bioanalyzer DNA1000 or FragmentAnalyzer, quantifiedbyqPCR (KAPAKK4835) and sequenced 1 * 51 + 10 + 10 bp on Illumina’s NextSeq2000.

    Purification:

    Article Title: Exploring contributions and responses of microbial quantity and diversity to coastal seasonal hypoxia.
    Article Snippet: Dissolved oxygen (DO) concentrations in euphotic waters are governed by combined effects from air-sea exchange, photosynthesis, and aerobic respiration.. Bacteria and microeukaryotes both respond to and shape these oxygen dynamics, yet the quantitative contributions of microbial traits versus physicochemical factors to DO variability remain unclear.. Moreover, the extent to which DO, and other environmental parameters or interdomain biotic interactions control microbial diversity and abundance is still unresolved.

    Gel Extraction:

    Article Title: Exploring contributions and responses of microbial quantity and diversity to coastal seasonal hypoxia.
    Article Snippet: Dissolved oxygen (DO) concentrations in euphotic waters are governed by combined effects from air-sea exchange, photosynthesis, and aerobic respiration.. Bacteria and microeukaryotes both respond to and shape these oxygen dynamics, yet the quantitative contributions of microbial traits versus physicochemical factors to DO variability remain unclear.. Moreover, the extent to which DO, and other environmental parameters or interdomain biotic interactions control microbial diversity and abundance is still unresolved.

    Generated:

    Article Title: Exploring contributions and responses of microbial quantity and diversity to coastal seasonal hypoxia.
    Article Snippet: Dissolved oxygen (DO) concentrations in euphotic waters are governed by combined effects from air-sea exchange, photosynthesis, and aerobic respiration.. Bacteria and microeukaryotes both respond to and shape these oxygen dynamics, yet the quantitative contributions of microbial traits versus physicochemical factors to DO variability remain unclear.. Moreover, the extent to which DO, and other environmental parameters or interdomain biotic interactions control microbial diversity and abundance is still unresolved.

    High Molecular Weight:

    Article Title: Genome Sequencing and Genome-Wide Analysis Uncover Candidate Genes and Loci Linked to Key Production Traits in the Philippine Black Tiger Shrimp (Penaeus monodon).
    Article Snippet: Penaeus monodon (black tiger shrimp) is a key species in aquaculture globally and in the Philippines.. The selection and development of stocks with desirable production traits, such as enhanced growth and disease resistance, are critical for improving production.. In this study, we leveraged a newly assembled, highquality genome sequence from a Philippine population to perform genomewide scans for SNPs associated with key production traits.

    Sequencing:

    Article Title: The pan-genome provides insights into evolutionary dynamics and fatty acid metabolism in Aceraceae family.
    Article Snippet: .. Library preparation was performed using the Illumina TruSeq DNA PCR-Free library kit to ensure high-quality, adapter-ligated fragments suitable for sequencing. .. The prepared libraries were then subjected to paired-end sequencing using the Illumina HiSeq X Ten platform, producing high-coverage (average 30x) reads.

    Article Title: Centromeric retrotransposons shape chromosomal evolution beyond pericentromeric regions.
    Article Snippet: .. Short-read whole-genome sequencing (used for polishing ONT contigs) was performed at the Next Generation Sequencing (NGS) facility of Bern, Switzerland (https://www.ngs.unibe.ch/), using the Illumina TruSeq DNA PCR-free kit for library preparation and 150bp paired-end reads (insert size of 550 bp) for sequencing on Illumina NovaSeq 6000 (estimated sequencing depth of 40X). .. Hi-C sequencing of three species (B. varia, B. prealpina and B. frutescens) was carried out by Phase Genomics company (Seattle, USA).

    Article Title: A grafting-rescued albino mutant reveals the essential function of CspTAC12 in cucumber chloroplast biogenesis.
    Article Snippet: Chloroplast biogenesis requires precise coordination between the nuclear and plastid genomes, orchestrated by the plastid-encoded RNA polymerase (PEP) complex.. In this study, we characterized a spontaneous albino lethal mutant, XNS106_1, in cucumber.. The mutant exhibits complete chlorophyll deficiency and photosynthetic failure, leading to post-germinative lethality.

    Next-Generation Sequencing:

    Article Title: Centromeric retrotransposons shape chromosomal evolution beyond pericentromeric regions.
    Article Snippet: .. Short-read whole-genome sequencing (used for polishing ONT contigs) was performed at the Next Generation Sequencing (NGS) facility of Bern, Switzerland (https://www.ngs.unibe.ch/), using the Illumina TruSeq DNA PCR-free kit for library preparation and 150bp paired-end reads (insert size of 550 bp) for sequencing on Illumina NovaSeq 6000 (estimated sequencing depth of 40X). .. Hi-C sequencing of three species (B. varia, B. prealpina and B. frutescens) was carried out by Phase Genomics company (Seattle, USA).

    Biomarker Discovery:

    Article Title: A grafting-rescued albino mutant reveals the essential function of CspTAC12 in cucumber chloroplast biogenesis.
    Article Snippet: Chloroplast biogenesis requires precise coordination between the nuclear and plastid genomes, orchestrated by the plastid-encoded RNA polymerase (PEP) complex.. In this study, we characterized a spontaneous albino lethal mutant, XNS106_1, in cucumber.. The mutant exhibits complete chlorophyll deficiency and photosynthetic failure, leading to post-germinative lethality.



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